L-蘋果酸檢測試劑盒

發表于由

L-蘋果酸檢測試劑盒L-Malic Acid Assay Kit (Manual Format)

產品貨號： K-LMALL

產品品名： L- 蘋果酸檢測試劑盒（大）

英文品名： L-Malic Acid Assay Kit (Large)

規格型號： 116 assays

原理：

反應時間： 3min

檢測限： 0.25 mg/L,

適用樣品： 飲料和食品以及其他原料

優點：

l ?? 成本低

l ?? 使用期間所有試劑的穩定性 >2 年

l ?? 操作簡單

l ?? 包含標準品

REFERENCES:

  1. Mollering, H. (1985). L-Malate. In? Methods of Enzymatic Analysis? (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
  2. AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
  3. Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples.? Zeitschrift fur Lebensmittel-Unterschung und-Forschung,? 162, 259-261.
  4. Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer.? J. Inst. Brew.? 92, 225-228.
  5. Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study.? J. AOAC Int . 77, 411-415.
商品說明：

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format