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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
Goat Anti-Biotin, Peroxidase Conjugated
Description
Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 1 mg active conjugate/ml |
| Recommended Storage | 2-8 °C, DO NOT FREEZE |
| Solution | 10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal |
| Recommended Usage | Moles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 – 5.0 μg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. |
| Detection Target | Biotin |
| Conjugate | Peroxidase |
| Host Species | Goat |
| Format | Concentrate |
| Safety Title | WARNING: Cancer – www.P65Warnings.ca.gov |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Biotin (Long Arm) Hydrazide Catalog #: SP-1100-50
Biotin (Long Arm) NHS Catalog #: SP-1200-50
Biotin (Long Arm) NHS, Water Soluble Catalog #: SP-1210-50
Goat Anti-Biotin, Unconjugated Catalog #: SP-3000-1
Citations
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Our high affinity polyclonal anti-biotin antibody is produced by hyperimmunizing goats with biotin and is purified by affinity chromatography. Anti-Biotin is available unconjugated, bound to heat stable, cross-linked agarose beads or labeled with enzymes or fluorescein. Agarose bound Anti-Biotin contains 1 mg of Anti-Biotin per ml of settled 4% agarose gel. This product can be used to bind and dissociate biotinylated molecules. Fluorescein labeled Anti-Biotin has been optimally conjugated to provide a bright fluorescent signal. The horseradish peroxidase and alkaline phosphatase conjugates have been produced with the highest specific activity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody was chosen to provide stable, highly active conjugates for immunohistochemical, enzyme immunoassay, or transfer blot applications.
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