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ImmPACT? Vector? Red Substrate, Alkaline Phosphatase (AP)
Description
ImmPACT Vector?Red AP Substrate Kit?produces a magenta?reaction product that is crisper and is 2-4 times more sensitive than the original Vector? Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
Features:
- Ideal for IHC/ICC/ISH and blots
- Fluorescent
- Heat Stable
- Permanent mounting
- Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
- Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
- Emission: Above 560 nm
- Color: Red
Specifications
| Unit Size | 120 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Magenta |
| Color of Fluorescence | Red |
| Microscopy | Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 120 ml ImmPACT Vector Red Diluent
- 2.5 ml ImmPACT Vector Red Reagent 1
- 2.5 ml ImmPACT Vector Red Reagent 2
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL? Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Hematoxylin Counterstain Catalog #: H-3401-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
Methyl Green Counterstain Catalog #: H-3402-500
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VectaMount? Permanent Mounting Medium Catalog #: H-5000-60
VectaMount? AQ Aqueous Mounting Medium Catalog #: H-5501-60
Levamisole Solution Catalog #: SP-5000-18
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
Citations
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Both Vector Red and ImmPACT Vector Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector Red or ImmPACT Vector Red AP chromogen can be dehydrated, cleared, and permanently mounted. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. Both formulations can be used for either manual or automated staining methods.
The bright fluorescence of the Vector Red or ImmPACT Vector Red reaction products are visible using DyLight? 594, Texas Red? or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.
Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector Red and ImmPACT Vector Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.
