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ImmPRESS? Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta)
Description
This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit?IgG and anti-mouse?IgG affinity-purified, highly cross-adsorbed secondary antibodies.
Features:
Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.
- Complete Kits for IHC Double Labeling
- Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
- High Sensitivity and Specificity
- Non-Biotin Based Reagents
- Prediluted, Ready-to-use
Kit Contents:
- 15 ml BLOXALL? Blocking Solution
- 15 ml 2.5% Normal Serum
- 15 ml ImmPRESS Duet HRP/AP Polymer Reagent?(mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS ?AP Anti-Mouse IgG)
- ImmPACT??DAB EqV Substrate (HRP, brown)
- ImmPACT Vector? Red Substrate (AP, magenta)
Note:?The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.
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Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry |
| Technology | Micropolymer Reagents |
| Detection Enzyme(s) | HRP/AP |
| Conjugate | Micropolymer HRP/Micropolymer AP |
| Target Species | Universal (Mouse/Rabbit) |
| Format | Ready-to-Use |
| Blocking Action | Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking |
Documents
- Safety Data Sheet
- User Guide
- ImmPRESS Polymer & Automated Staining App. Note
- ImmPRESS Troubleshooting Guide
- Download CoA
- Datasheet
Related Products
ImmPACT? Vector? Red Substrate, Alkaline Phosphatase (AP) Catalog #: SK-5105
Vector? Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300
BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400
ImmPRESS?-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent Catalog #: MP-6402-15
ImmPRESS? Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) Catalog #: MP-7724
ImmPRESS? HRP Goat Anti-Rat IgG Polymer Detection Kit, Peroxidase Catalog #: MP-7404-50
ImmPRESS? HRP Horse Anti-Mouse IgG, Rat adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7422-15
ImmPRESS? HRP Goat Anti-Rat IgG, Mouse adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7444-15
Normal Horse Serum Blocking Solution, 2.5% Catalog #: S-2012-50
ImmPACT? DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105
ImmPACT? VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605
ImmPACT? SG Substrate, Peroxidase (HRP) Catalog #: SK-4705
ImmPACT? NovaRED? Substrate, Peroxidase (HRP) Catalog #: SK-4805
ImmPACT? AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205
Technical Information
Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.? These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector? Red (AP, magenta) substrates are included to complete this easy to use staining kit.
Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.
Also included, BLOXALL ?Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.
All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.
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Learn more about the ImmPRESS Polymer Detection Systems in double labeling.
Product review on Biocompare.com.
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