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Dolichos Biflorus Agglutinin (DBA), Fluorescein
Description
Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. ?It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Fluorescein labeled?Dolichos biflorus?agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
| Unit Size | 2 mg, 5 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 |
| Concentration | FL-1031-2 (2 mg active conjugate/ml) FL-1031-5 (5 mg active conjugate/ml) |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | N-Acetylgalactosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Dolichos Biflorus Agglutinin (DBA), Unconjugated Catalog #: L-1030-5
Dolichos Biflorus Agglutinin (DBA), Rhodamine Catalog #: RL-1032-2
N-acetylgalactosamine Catalog #: S-9001
Dolichos Biflorus Agglutinin (DBA), Biotinylated Catalog #: B-1035-5
Citations
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Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine