NISSUI日水RPMI 1640 Medium
Directions
Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.
Summary
RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.
Formula
|
Components
|
In 10.2 g/L
|
|
L-Arginine Hydrochloride
|
240.0 mg
|
|
L-Asparagine, H2O
|
56.8 mg
|
|
L-Asparatic Acid
|
20.0 mg
|
|
L-Cysteine Hydrochloride, H2O
|
72.9 mg
|
|
L-Glutamic Acid
|
20.0 mg
|
|
Succinic Acid
|
46.0 mg
|
|
Sodium Succinate,6 H2O
|
164.0 mg
|
|
Glutathione
|
1.0 mg
|
|
Glycine
|
10.0 mg
|
|
L-Histidine Hydrochloride, H2O
|
20.3 mg
|
|
L-Hydroxyproline
|
20.0 mg
|
|
L-Isoleucine
|
50.0 mg
|
|
L-Leucine
|
50.0 mg
|
|
L-Lysine Hydrochloride
|
40.0 mg
|
|
L-Methionine
|
15.0 mg
|
|
L-Phenylalanine
|
15.0 mg
|
|
L-Proline
|
20.0 mg
|
|
L-Serine
|
30.0 mg
|
|
L-Threonine
|
20.0 mg
|
|
L-Tryptophan
|
5.0 mg
|
|
L-Tyrosine
|
20.0 mg
|
|
L-Valine
|
20.0 mg
|
|
Biotin
|
0.2 mg
|
|
Calcium Pantothenate
|
0.25 mg
|
|
Choline Chloride
|
3.0 mg
|
|
Folic Acid
|
1.0 mg
|
|
Inositol
|
35.0 mg
|
|
Nicotinamide
|
1.0 mg
|
|
p-Aminobenzoic Acid
|
1.0 mg
|
|
Pyridoxine Hydrochloride
|
1.0 mg
|
|
Riboflavin
|
0.2 mg
|
|
Thiamin Hydrochloride
|
1.0 mg
|
|
Cyanocobalamin
|
0.005 mg
|
|
Sodium Chloride
|
6,000.0 mg
|
|
Potassium Chloride
|
400.0 mg
|
|
Calcium Nitrate
|
69.5 mg
|
|
Monosodium Phosphate
|
677.0 mg
|
|
Magnesium Sulfate
|
48.8 mg
|
|
Glucose
|
2,000.0 mg
|
|
Phenol Red
|
5.0 mg
|
00883 NISSUI 日本日水?RPMI 1640液體培養基RPMI 1640 Liquid
產品編號：00883
中文名稱：RPMI 1640液體培養基
英文品名：RPMI 1640 Liquid
包裝規格：500ml
用途：液態組織培養基
使用方法:
RPMI 1640 Liquid“日水”是根據由RPMI（Roswell Park Memorial Institute）的Director即Dr.George E.Moore開發出來的浮游細菌培養基制造而成的液態培養基。本培養基被用作老鼠和人的白血病細胞的第一代培養基以及株的第二代培養基。
在使用前，請根據不同目的添加適量血清。
組成（mg/L):
RPMI 1640液體培養基RPMI 1640 Liquid編號：00883?
硝酸鈣（四水鹽）……………100
氯化鉀…………………………400
硫酸鎂（無水）………………48.84
氯化鈉…………………………6000
磷酸一氫二鈉（無水）………800
L-精氨酸鹽酸鹽………………200
L-天門冬酰胺…………………50
L-天冬氨酸……………………20
RPMI 1640液體培養基RPMI 1640 Liquid編號：00883?
L-胱氨酸二鹽酸鹽……………65.15
L-谷氨酸………………………20
L-谷氨酰胺……………………300
甘氨酸…………………………10
L-組氨酸………………………15
L-羥脯氨酸……………………20
L-異亮氨酸……………………50
L-賴氨酸鹽酸鹽………………40
L-甲硫氨酸……………………15
L-苯基丙氨酸…………………15
L-脯氨酸………………………20
L-絲氨酸………………………30
L-蘇氨酸………………………20
L-色氨酸………………………5
L-酷氨酸二鈉（二水鹽）……28.83
L-纈氨酸………………………20
生物素…………………………0.2
泛酸鈣…………………………0.25
氯化膽堿………………………3
RPMI 1640液體培養基RPMI 1640 Liquid編號：00883?
葉酸……………………………1
肌醇……………………………35
煙酰胺…………………………1
鹽酸吡哆醛……………………1
核黃酸………………………0.2
鹽酸硫胺………………………1
維他命B12……………………0.005
D．葡萄糖……………………2000
對氨基苯甲酸…………………1
谷胱甘肽（還原型）…………1
酚紅（鈉鹽）………………5.3
碳酸氫鈉……………………2000
貯藏方法、使用期限:
保存在低溫（2~8℃）之處。使用期限為一年。