Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.
DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.
Unit Size | 0.5 mg |
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Applications | Immunofluorescence, In situ hybridization |
Concentration | 1 mg active conjugate/ml |
Recommended Storage | 2-8 °C |
Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide |
Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. |
Detection Target | Streptavidin |
Conjugate | DyLight 488 |
Color of Fluorescence | Green |
Host Species | Goat |
Format | Concentrate |
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Anti-Streptavidin Antibody, Unconjugated Catalog #: SP-4000-1
Anti-Avidin Antibody, Biotinylated Catalog #: BA-0300-.5
The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.
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The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody?is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Unit Size | 50 ml |
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Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
Concentration | Ready-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
Recommended Storage | 2-8 °C |
Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN? Elite? ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. |
Target Species | Goat |
Conjugate | Biotinylated |
Host Species | Horse |
Format | Ready-to-Use |
VECTASTAIN? Elite ABC-HRP Reagent, Peroxidase, R.T.U. Catalog #: PK-7100
Normal Horse Serum Blocking Solution, 2.5% Catalog #: S-2012-50
Texas Red? Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1
VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Normal Horse Serum Blocking Solution Catalog #: S-2000-20
Streptavidin, DyLight? 488 Catalog #: SA-5488-1
Streptavidin, DyLight? 549 Catalog #: SA-5549-1
Streptavidin, DyLight? 594 Catalog #: SA-5594-1
Streptavidin, DyLight? 649 Catalog #: SA-5649-1
Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100
Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker? solution (Cat. No. SP-5030) is recommended.
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VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight?fluorescent dyes in a single step. ?Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight?dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.
Unit Size | 1 kit |
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Applications | Immunofluorescence |
Target Species | Universal (Mouse/Rabbit) |
Conjugate | DyLight 488/DyLight 594 |
Color of Fluorescence | Kit (Green, Red) |
Host Species | Horse |
Format | Ready-to-Use |
Kit Contents:
DyLight? 488:
DyLight?594:
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Kit Contents:
DyLight?488:
DyLight?594:
DyLight?fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?
DyLight?dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight?dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight?dyes can be applied as single labels or in combination with other DyLight?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight?dyes currently offered are DyLight?488 (green), DyLight?549 (orange), DyLight?594 (red), and DyLight?649 (far red).
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]]>WHEATON 染色皿盖子 3个/包_染色皿_WHEATON_玻璃制品
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VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight?fluorescent dyes in a single step. ?Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight?dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.
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Unit Size | 1 kit |
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Applications | Immunofluorescence |
Target Species | Universal (Mouse/Rabbit) |
Conjugate | DyLight 488/DyLight 594 |
Color of Fluorescence | Kit (Green, Red) |
Host Species | Horse |
Format | Ready-to-Use |
Kit Contents:
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Kit Contents:
DyLight? 488:
DyLight? 594:
DyLight?fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?
DyLight?dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight?dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight?dyes can be applied as single labels or in combination with other DyLight?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight?dyes currently offered are DyLight?488 (green), DyLight?549 (orange), DyLight?594 (red), and DyLight?649 (far red).
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